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Tested Applications
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67594 targets NOXA2/p67phox in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NOXA2/p67phox fusion protein Ag8691 Predict reactive species |
| Full Name | neutrophil cytosolic factor 2 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC001606 |
| Gene Symbol | NCF2 |
| Gene ID (NCBI) | 4688 |
| RRID | AB_3084362 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P19878 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NOXA2/p67phox is also named as NCF2(Neutrophil cytosol factor 2) and belongs to the NCF2/NOXA1 family. It is a membrane bound cytochrome b558 required for activation of the latent NADPH oxidase. NCF2 is an essential component of the multi-protein NADPH oxidase enzyme in phagocytic leukocytes, as well as in certain non-phagocytic cells. This protein has 4 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NOXA2/p67phox antibody CL488-67594 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
