NOXA2/p67phox Monoclonal antibody, PBS Only

NOXA2/p67phox Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D11A3

Cat no : 67594-1-PBS

Synonyms

FLJ93058, NADPH oxidase activator 2, NCF 2, NCF2, Neutrophil cytosol factor 2, neutrophil cytosolic factor 2, NOXA2, P67 PHOX, P67PHOX

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 67594-1-Ig (same clone as 67594-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67594-1-Ig (NOXA2/p67phox antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67594-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 67594-1-Ig (same clone as 67594-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 67594-1-Ig (NOXA2/p67phox antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67594-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 67594-1-Ig (same clone as 67594-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67594-1-Ig (NOXA2/p67phox antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67594-1-Ig (same clone as 67594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67594-1-Ig (NOXA2/p67phox antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67594-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67594-1-Ig (same clone as 67594-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using NOXA2/p67phox antibody (67594-1-Ig, Clone: 1D11A3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67594-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67594-1-PBS targets NOXA2/p67phox in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NOXA2/p67phox fusion protein Ag8691
• Full Name: neutrophil cytosolic factor 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC001606
• Gene Symbol: NCF2
• Gene ID (NCBI): 4688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.