NOX4 Monoclonal antibody

NOX4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

CloneNo.

4E5F1

Cat no : 67681-1-Ig

Synonyms

Kidney oxidase 1, KOX, KOX 1, NADPH oxidase 4, NOX4, Renal NAD(P)H oxidase, RENOX

---

Validation Data Gallery

• 

  WB analysis using 67681-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67681-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-87 MG using 67681-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67681-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 67681-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67681-1-Ig (NOX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 67681-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67681-1-Ig (NOX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 67681-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using NOX4 antibody (67681-1-Ig, Clone: 4E5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HUVEC using 67681-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using NOX4 antibody (67681-1-Ig, Clone: 4E5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, U-87 MG cells, HSC-T6 cells, HepG2 cells, HeLa cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

67681-1-Ig targets NOX4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NOX4 fusion protein Ag6176
• Full Name: NADPH oxidase 4
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC040105
• Gene Symbol: NOX4
• Gene ID (NCBI): 50507
• RRID: AB_2882874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOX4 (NADPH oxidase 4) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and it has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex. Studies have reported molecular masses of Nox4 protein by western blot analysis ranging from 55 to 80 kDa. The truncated NOX4 splice variant D (28 kDa) lacks the majority of the transmembrane domain and has been shown to produce higher levels of ROS and DNA damage compared to its prototype. NOX4D has previously been shown to localise to the nucleus and nucleolus in various cell types and is implicated in the generation of reactive oxygen species (ROS) and DNA damage (PMID: 11728818, PMID: 29285262, PMID: 14670934). Nox4 in cardiac myocytes is primarily expressed in mitochondria, and upregulation of Nox4 induced by hypertrophic stimuli elicits mitochondrial dysfunction and cardiac failure. In breast or ovarian tumor cells, mitochondrial Nox4 contributes to oncogenesis. In vascular endothelial cells, however, Nox4 is expressed in the endoplasmic reticulum (ER) and plays a specific role in redox-mediated ER signaling (PMID: 24259511).

Publications

Species	Application	Title
human	WB	Biomedicines; Identification of NOX4 as a New Biomarker in Hepatocellular Carcinoma and Its Effect on Sorafenib Therapy; Authors - Hui-Zhou Li
human	WB	Exp Biol Med (Maywood); Triptolide decreases podocytes permeability by regulating TET2-mediated hydroxymethylation of ZO-1; Authors - Yue-Wen Tang
mouse	WB,IP,IF	J Pineal Res; Melatonin Alleviates Osteoarthritis by Regulating NADPH Oxidase 4-Induced Ferroptosis and Mitigating Mitochondrial Dysfunction; Authors - Qi Wang ; KD Validated
mouse	WB	Mech Ageing Dev; Deletion of Stimulator of Interferons Genes Aggravated Cardiac Dysfunction in Physiological Aged Mice; Authors - Diansa Gao
human	IF	Antioxidants (Basel); Sirtuin1 Mediates the Protective Effects of Echinacoside against Sepsis-Induced Acute Lung Injury via Regulating the NOX4-Nrf2 Axis; Authors - Weixi Xie
mouse	WB	Int Immunopharmacol; PLC-CN-NFAT1 signaling-mediated Aβ and IL-1β crosstalk synergistically promotes hippocampal neuronal damage; Authors - Qifeng Shi