NOX3 Polyclonal antibody

NOX3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 30131-1-AP

Synonyms

GP91 3, gp91phox homolog 3, Mitogenic oxidase 2, MOX 2, MOX2, NADPH oxidase 3, NOX3

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Validation Data Gallery

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  WB analysis using 30131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30131-1-AP (NOX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, HEK-293 cells, PC-12 cells, mouse kidney tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

30131-1-AP targets NOX3 in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOX3 fusion protein Ag32601
• Full Name: NADPH oxidase 3
• Calculated Molecular Weight: 64kd
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: NM_015718
• Gene Symbol: NOX3
• Gene ID (NCBI): 50508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOX3 is a member of NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. NOX3 plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. The antibody is specific to NOX3.

Publications

Species	Application	Title
pig	WB	J Thorac Cardiovasc Surg; Effects of Diet-induced Metabolic Syndrome on Cardiac Function and Angiogenesis in Response to Sodium-glucose Cotransporter-2 Inhibitor Canagliflozin; Authors - Dwight D Harris