NOV Polyclonal antibody

NOV Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55449-1-AP

Synonyms

CCN family member 3, CCN3, IBP 9, IGFBP 9, IGFBP9, NOV

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Validation Data Gallery

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  WB analysis of mouse liver using 55449-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55449-1-AP (NOV antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human nephroblastoma using 55449-1-AP

  Immunohistochemical analysis of paraffin-embedded human nephroblastoma using 55449-1-AP (NOV antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 55449-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 55449-1-AP (NOV antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 55449-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 55449-1-AP (NOV antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human nephroblastoma tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

55449-1-AP targets NOV in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nephroblastoma overexpressed gene
• Calculated Molecular Weight: 39 kDa
• GenBank Accession Number: NM_002514
• Gene Symbol: NOV
• Gene ID (NCBI): 4856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	J Cell Mol Med; NOV/CCN3 induces cartilage protection by inhibiting PI3K/AKT/mTOR pathway.; Authors - Xiaojian Huang