NOTUM Monoclonal antibody, PBS Only (Detector)

NOTUM Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F6D5

Cat no : 66956-1-PBS

Synonyms

Protein notum homolog, Palmitoleoyl-protein carboxylesterase NOTUM, OK/SW-CL.30, hNOTUM, EC:3.1.1.98

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Validation Data Gallery

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  WB analysis using 66956-1-Ig (same clone as 66956-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66956-1-Ig (same clone as 66956-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66956-1-Ig (same clone as 66956-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 66956-1-Ig (same clone as 66956-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66956-1-Ig (same clone as 66956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66956-1-Ig (NOTUM antibody) at dilution of 1:5000 (under 10x lens).. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66956-1-Ig (same clone as 66956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66956-1-Ig (NOTUM antibody) at dilution of 1:5000 (under 40x lens).. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50968-3

  Cytometric bead array standard curve of MP50968-3, NOTUM Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66956-2-PBS. Detection antibody: 66956-1-PBS. Standard:Ag6338. Range: 0.098-100 ng/mL.

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Product Information

66956-1-PBS targets NOTUM as part of a matched antibody pair:

MP50968-3: 66956-2-PBS capture and 66956-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NOTUM fusion protein Ag6338
• Full Name: notum pectinacetylesterase homolog (Drosophila)
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC060882
• Gene Symbol: NOTUM
• Gene ID (NCBI): 147111
• RRID: AB_2882279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.