NOTCH3 Polyclonal antibody

NOTCH3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 30540-1-AP

Synonyms

CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila), NOTCH3

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Validation Data Gallery

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  WB analysis using 30540-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30540-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 30540-1-AP

  IP result of anti-NOTCH3 (IP:30540-1-AP, 4ug; Detection:30540-1-AP 1:1000) with HeLa cells lysate 1320 ug.

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Tested Applications

• Positive WB detected in: Caco-2 cells, HeLa cells, U2OS cells, mouse lung tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30540-1-AP targets NOTCH3 in WB, IP, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOTCH3 fusion protein Ag30242
• Full Name: Notch homolog 3 (Drosophila)
• Calculated Molecular Weight: 244 kDa
• Observed Molecular Weight: 270 kDa, 90 kDa
• GenBank Accession Number: NM_000435
• Gene Symbol: NOTCH3
• Gene ID (NCBI): 4854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOTCH3 (Neurogenic locus notch homolog protein 3) is one of four mammalian Notch proteins, which act as signalling receptors to control cell fate in many developmental and adult tissue contexts (PMID: 32210034). Notch is a transmembrane, developmental signalling receptor, which plays many crucial roles in developmental patterning, cell fate decisions, regulation of cell survival and proliferation (PMID:30246579). Genetic studies have shown that Notch3 gene knockouts are viable and have limited developmental defects, focussed mostly on defects in the arterial smooth muscle cell lineage. Notch3, in collaboration with other Notch proteins, is involved in stem cell regulation in different tissues in stem cell regulation in different tissues, and it also controls the plasticity of the vascular smooth muscle phenotype involved in arterial vessel remodelling. Overexpression, gene amplification and mis-activation of Notch3 are associated with different cancers, in particular triple negative breast cancer and ovarian cancer (PMID: 32210034).