NOSIP Polyclonal antibody

NOSIP Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27979-1-AP

Synonyms

CGI 25, eNOS interacting protein, NOSIP

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Validation Data Gallery

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  WB analysis using 27979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 27979-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 27979-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 27979-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung using 27979-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 27979-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27979-1-AP (NOSIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, A431 cells, A549 cells, Ramos cells, HepG2 cells, L02 cells
• Positive IHC detected in: human colon tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

27979-1-AP targets NOSIP in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOSIP fusion protein Ag27569
• Full Name: nitric oxide synthase interacting protein
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC010077
• Gene Symbol: NOSIP
• Gene ID (NCBI): 51070
• RRID: AB_2881027
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	iScience; Nosip is a potential therapeutic target in hepatocellular carcinoma cells; Authors - Junjie Gao ; KD Validated