NOP58 Recombinant antibody, PBS Only

NOP58 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230058C7

Cat no : 82879-1-PBS

Synonyms

Nucleolar protein 5, NOP5/NOP58, NOP5, NOL5, HSPC120

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Validation Data Gallery

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  WB analysis using 82879-1-RR (same clone as 82879-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82879-1-RR (NOP58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 82879-1-RR (same clone as 82879-1-PBS)

  IP result of anti-NOP58 (IP:82879-1-RR, 4ug; Detection:82879-1-RR 1:2000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 82879-1-RR (same clone as 82879-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82879-1-RR (NOP58 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 82879-1-RR (same clone as 82879-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82879-1-RR (NOP58 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 82879-1-RR (same clone as 82879-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOP58 antibody (82879-1-RR) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82879-1-RR (same clone as 82879-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NOP58 Recombinant antibody (82879-1-RR, Clone:230058C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82879-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 82879-1-RR against Human NOP58 were performed. The affinity constant is below 1 pM.

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Product Information

82879-1-PBS targets NOP58 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NOP58 fusion protein Ag5740
• Full Name: NOP58 ribonucleoprotein homolog (yeast)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC032592
• Gene Symbol: NOP58
• Gene ID (NCBI): 51602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The box C/D snoRNPs are named for the short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. The box C/D sequences are also required for multiple aspects. of snoRNA biogenesis, including RNA stability, intronic processing, nucleolar targeting, nuclear retention, and 59 trimethylguanosine cap formation (PMID:9649444,8878486). Nop58 is a component common to the box C/D small nucleolar ribonucleoprotein (PMID:10606270).