NOP2 Monoclonal antibody

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  WB analysis using 68889-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68889-1-Ig (NOP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 68889-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOP2 antibody (68889-1-Ig, Clone: 1F4F4 ) at dilution of 1:561 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005).

NOP2 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68889-1-Ig

Clone No.1F4F4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Nucleolar protein 1, NSUN1, NOP120, NOL1, EC:2.1.1.-

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, Daudi cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:280-1:1122

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68889-1-Ig targets NOP2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NOP2 fusion protein Ag33985
• Full Name: NOP2 nucleolar protein homolog (yeast)
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC000656
• Gene Symbol: NOP2
• Gene ID (NCBI): 4839
• RRID: AB_3670447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P46087
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOL1, (synonyms: p120, NSUN1, NOP120), is a 120 kDa proliferating-cell nucleolar antigen and is the most cancer specific of the proliferation-associated nucleolar proteins identified thus far. NOL1 is expressed in G1 and peaks during the early S phase of the cell cycle and it has not been detected in benign tumors and most normal resting tissues. Overexpression of NOL1 caused the transformation of NIH 3T3 cells and expression of an antisense NOL1 construct inhibited the growth of NIH 3T3 cells. NOL is localized in a novel nucleolar microfibrillar structure, and contains, consecutively, four major domains: a basic domain, an acidic domain, a hydrophobic and methionine-rich domain, and a domain rich in cysteine and proline residues. The gene for human NOL1 was assigned to chromosome 12p13 (PMID: 23775790).