NOM1 Polyclonal antibody

• 

  WB analysis using 29798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29798-1-AP (NOM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 29798-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29798-1-AP (NOM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 29798-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29798-1-AP (NOM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NOM1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 29798-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

C7orf3, FLJ16401, NOM1, SGD1, SGD1 homolog

---

Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, SW 1990 cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29798-1-AP targets NOM1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOM1 fusion protein Ag31423
• Full Name: nucleolar protein with MIF4G domain 1
• Calculated Molecular Weight: 96KD
• Observed Molecular Weight: 96~100 kDa
• GenBank Accession Number: NM_138400
• Gene Symbol: NOM1
• Gene ID (NCBI): 64434
• RRID: AB_3086164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5C9Z4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleolar protein NOM1, which contains an MIF4G domain and an MA3 domain, was first isolated from the bone marrow of children with acute myeloid leukemia. NOM1 is highly conserved in a variety of species, including in yeast and in humans.