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Tested Applications
| Positive WB detected in | COLO 320 cells, Jurkat cells |
| Positive IP detected in | COLO 320 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12043-1-AP targets NOL8 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NOL8 fusion protein Ag2672 Predict reactive species |
| Full Name | nucleolar protein 8 |
| Calculated Molecular Weight | 132 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC013788 |
| Gene Symbol | NOL8 |
| Gene ID (NCBI) | 55035 |
| RRID | AB_2152001 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q76FK4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NOL8 (Nucleolar Protein 8) plays an essential role in the survival of diffuse-type gastric cancer cells,and knocking down NOL8 promoted cell apoptosis. The structure analysis suggested that NOL8 contains a RNA binding motif, which was consistent with the study that NOL8 interacted with DEAD-box RNA helicase
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NOL8 antibody 12043-1-AP | Download protocol |
| IP protocol for NOL8 antibody 12043-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
