NOC2L Polyclonal antibody, PBS Only

NOC2L Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28509-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 28509-1-AP (same clone as 28509-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28509-1-AP (NOC2L antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28509-1-PBS in a different storage buffer formulation.

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  WB analysis using 28509-1-AP (same clone as 28509-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28509-1-AP (NOC2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28509-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 28509-1-AP (same clone as 28509-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28509-1-AP (NOC2L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28509-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 28509-1-AP (same clone as 28509-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28509-1-AP (NOC2L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28509-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 28509-1-AP (same clone as 28509-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 28509-1-AP (NOC2L antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Phalloidine stains the F-actin (red). This data was developed using the same antibody clone with 28509-1-PBS in a different storage buffer formulation.

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Product Information

28509-1-PBS targets NOC2L in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOC2L fusion protein Ag26952
• Full Name: nucleolar complex associated 2 homolog (S. cerevisiae)
• Calculated Molecular Weight: 749 aa, 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC003555
• Gene Symbol: NOC2L
• Gene ID (NCBI): 26155
• RRID: AB_2881160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NOC2L, also known as NIR, is a transcriptional corepressor with inhibitor of histone acetyltransferase (INHAT) activity (PMID: 16322561). It prevents acetylation of all core histones by the histone acetyltransferase EP300/p300. NOC2L interacts with p53, inhibits p53-activated gene expression, and regulates p53-dependent apoptosis (PMID: 16322561). NIR binds to TAp63 and acts as a repressor of TAp63-mediated transactivation of the p21Cip1 gene (PMID: 20123734). NOC2L is ubiquitously expressed throughout embryonic development and adulthood (PMID: 16322561). NOC2L has been reported as a key regulator of skin development. Epidermis-specific ablation of NOC2L impairs epidermal stratification and barrier function (PMID: 29490983).