NMT1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NMT1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D9B9

Cat no : 67984-1-PBS

Synonyms

N myristoyltransferase 1, NMT1, Type I N myristoyltransferase

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Validation Data Gallery

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  WB analysis using 67984-1-Ig (same clone as 67984-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67984-1-Ig (NMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67984-1-PBS in a different storage buffer formulation.

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  WB analysis of SKOV-3 using 67984-1-Ig (same clone as 67984-1-PBS)

  WB result of NMT1 antibody (67984-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NMT1 transfected SKOV-3 cells. This data was developed using the same antibody clone with 67984-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67984-1-Ig (same clone as 67984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67984-1-Ig (NMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67984-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67984-1-Ig (same clone as 67984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67984-1-Ig (NMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67984-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67984-1-Ig (same clone as 67984-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67984-1-Ig (NMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67984-1-PBS in a different storage buffer formulation.

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Product Information

67984-1-PBS targets NMT1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NMT1 fusion protein Ag20541
• Full Name: N-myristoyltransferase 1
• Calculated Molecular Weight: 496 aa, 57 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC006569
• Gene Symbol: NMT1
• Gene ID (NCBI): 4836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NMT1 is a N-myristoyltransferase responsible for the transfer of myristate from CoA to an amino-terminal glycine of many eukaryotic proteins, which facilitates the targeting of proteins to membrane surfaces and is essential for viability of the organism. Insertional mutagenesis of the Nmt1 gene in Saccharomyces cerevisiae causes recessive lethality. Humans and mice possess two distinct but structurally similar enzymes, NMT1 and NMT2, ubiquitously expressed in most human and mouse tissues. Western analysis revealed that there are 4 isoforms of NMT1 with apparent molecular masses ranging from 49 to 68 kDa. In cell fractionation studies, the 68-kDa NMT1 isoform and NMT2 were present in both membrane and cytoplasmic fractions, while the smaller NMT1 isoforms were predominantly cytoplasmic.