NMRAL1 Polyclonal antibody

NMRAL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15765-1-AP

Synonyms

HSCARG, NMRAL1, SDR48A1

---

Validation Data Gallery

• 

  WB analysis using 15765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15765-1-AP (NMRAL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15765-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 15765-1-AP (NMRAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 15765-1-AP

  IP result of anti-NMRAL1 (IP:15765-1-AP, 4ug; Detection:15765-1-AP 1:1500) with Jurkat cells lysate 1560 ug.

• 

  IHC staining of human liver using 15765-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15765-1-AP (NMRAL1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 15765-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15765-1-AP (NMRAL1 antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, LNCaP cells, MCF-7 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

15765-1-AP targets NMRAL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NMRAL1 fusion protein Ag8401
• Full Name: NmrA-like family domain containing 1
• Calculated Molecular Weight: 299 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC007364
• Gene Symbol: NMRAL1
• Gene ID (NCBI): 57407
• RRID: AB_2153154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Stamp2 controls macrophage inflammation through nicotinamide adenine dinucleotide phosphate homeostasis and protects against atherosclerosis.; Authors - Henrik ten Freyhaus
rat	IF	Diabetes Metab Syndr Obes; STAMP2 Attenuates Cardiac Dysfunction and Insulin Resistance in Diabetic Cardiomyopathy via NMRAL1-Mediated NF-κB Inhibition in Type 2 Diabetic Rats; Authors - Zhan Gao