NMNAT1 Polyclonal antibody

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  WB analysis using 28493-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28493-1-AP (NMNAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NMNAT1 Polyclonal Antibody for WB, ELISA

Cat No. 28493-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Synonyms

NaMN adenylyltransferase 1, NMNAT1, PNAT 1, PNAT1

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28493-1-AP targets NMNAT1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NMNAT1 fusion protein Ag29584
• Full Name: nicotinamide nucleotide adenylyltransferase 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC014943
• Gene Symbol: NMNAT1
• Gene ID (NCBI): 64802
• RRID: AB_3086056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HAN9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NMNAT1 is a member of the nicotinamide-nucleotide adenylyltransferases (NMNATs) which catalyze nicotinamide adenine dinucleotide (NAD) synthesis (PMID: 28445802). NMNAT is a central enzyme in NAD biosynthesis, catalyzing the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PMID: 17402747). NMNAT1 is widely expressed with the highest levels in skeletal muscle, heart, and kidney(PMID: 11027696). Mutations in NMNAT1 have been shown associated with the LCA9 form of the retinal degeneration pathology Leber's congenital amaurosis (PMID: 22842229, 22842230).

Publications

Species	Application	Title
human,mouse	WB	Nat Cardiovasc Res; Mitochondrial NAD+ deficiency in vascular smooth muscle impairs collagen III turnover to trigger thoracic and abdominal aortic aneurysm; Authors - Jingjing Zhang