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NMNAT1 Polyclonal antibody

NMNAT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11399-1-AP

Synonyms

NaMN adenylyltransferase 1, NMNAT1, PNAT 1, PNAT1



Tested Applications

Positive WB detected inhuman testis tissue, mouse testis tissue, pig heart tissue
Positive IHC detected inmouse skeletal muscle tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11399-1-AP targets NMNAT1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NMNAT1 fusion protein Ag1944
Full Name nicotinamide nucleotide adenylyltransferase 1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 28-32 kDa
GenBank Accession NumberBC014943
Gene Symbol NMNAT1
Gene ID (NCBI) 64802
RRIDAB_10699868
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NMNAT1 is a member of the nicotinamide-nucleotide adenylyltransferases (NMNATs) which catalyze nicotinamide adenine dinucleotide (NAD) synthesis (PMID: 28445802). NMNAT is a central enzyme in NAD biosynthesis, catalyzing the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PMID: 17402747). NMNAT1 is widely expressed with the highest levels in skeletal muscle, heart, and kidney(PMID: 11027696). Mutations in NMNAT1 have been shown associated with the LCA9 form of the retinal degeneration pathology Leber's congenital amaurosis (PMID: 22842229, 22842230).

Protocols

Product Specific Protocols
WB protocol for NMNAT1 antibody 11399-1-APDownload protocol
IHC protocol for NMNAT1 antibody 11399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Am Soc Nephrol

Pre-emptive Short-term Nicotinamide Mononucleotide Treatment in a Mouse Model of Diabetic Nephropathy.

Authors - Itaru Yasuda
human,mouseIF

Metabolism

Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.

Authors - Ya Li
humanWB

J Proteome Res

Reduced Nicotinamide Mononucleotide (NMNH) Potently Enhances NAD(+) and Suppresses Glycolysis, the TCA Cycle, and Cell Growth

Authors - Yan Liu
mouseIHC

Sci Rep

Nicotinamide mononucleotide ameliorates adriamycin-induced renal damage by epigenetically suppressing the NMN/NAD consumers mediated by Twist2

Authors - Kazuhiro Hasegawa
mouseWB

Food Funct

β-Nicotinamide mononucleotide supplementation prolongs the lifespan of prematurely aged mice and protects colon function in ageing mice

Authors - Yanrou Gu
mouse,ratWB

EMBO J

Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function

Authors - Quanjiang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Caitlin (Verified Customer) (09-17-2020)

I used this antibody on neuronal cell lysate for Western blotting. The antibody was able to detect the protein of interest in my lysate (NMNAT3).

  • Applications: Western Blot
  • Cell Tissue Type: Mouse neurons