NMI Polyclonal antibody

NMI Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31096-1-AP

Synonyms

N myc (and STAT) interactor, N myc and STAT interactor, N myc interactor, NMI

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Validation Data Gallery

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  WB analysis using 31096-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31096-1-AP (NMI antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31096-1-AP targets NMI in WB, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NMI fusion protein Ag34759
• Full Name: N-myc (and STAT) interactor
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC001268
• Gene Symbol: NMI
• Gene ID (NCBI): 9111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

N-Myc and STAT Interactor protein (NMI) is an interferon inducible protein that interacts with NMYC and CMYC (members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. It is widely involved in the process of tumor growth, progression, and metastasis.