NME6 Polyclonal antibody

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  WB analysis of LNCaP using 10250-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 10250-1-AP (NME6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 10250-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 10250-1-AP (NME6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10250-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10250-1-AP (NME6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10250-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10250-1-AP (NME6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NME6 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10250-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

IPIA ALPHA, NDK 6, NDP kinase 6, NME6

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Tested Applications

• Positive WB detected in: LNCaP cells, U2OS cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10250-1-AP targets NME6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NME6 fusion protein Ag0315
• Full Name: non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC001808
• Gene Symbol: NME6
• Gene ID (NCBI): 10201
• RRID: AB_2298600
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75414
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.