NME5 Polyclonal antibody

NME5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 12923-1-AP

Synonyms

IPIA beta, NDK H 5, NDP kinase homolog 5, NME5, Testis specific nm23 homolog

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Validation Data Gallery

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  WB analysis of mouse testis using 12923-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12923-1-AP (NME5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12923-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12923-1-AP (NME5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12923-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12923-1-AP (NME5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12923-1-AP

  IP result of anti-NME5 (IP:12923-1-AP, 4ug; Detection:12923-1-AP 1:500) with mouse testis tissue lysate 3920 ug.

• 

  IHC staining of mouse testis using 12923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12923-1-AP (NME5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12923-1-AP (NME5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue, human kidney tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Product Information

12923-1-AP targets NME5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NME5 fusion protein Ag3610
• Full Name: non-metastatic cells 5, protein expressed in (nucleoside-diphosphate kinase)
• Calculated Molecular Weight: 212 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC026182
• Gene Symbol: NME5
• Gene ID (NCBI): 8382
• RRID: AB_2152973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NME5(Nucleoside diphosphate kinase homolog 5) belongs to the NDK family. Its expression plays a critical role in spermiogenesis by increasing the cellular levels of GPX5 to eliminate reactive oxygen species. NME5 may have specific functions in the phosphotransfer network involved in spermiogenesis and flagellar movement.

Publications

Species	Application	Title
mouse	WB,IF	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
mouse	WB	J Hazard Mater; TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis.; Authors - Changying Li
human	IHC	Int J Cancer; Additive effect of the AZGP1, PIP, S100A8, and UBE2C molecular biomarkers improves outcome prediction in breast carcinoma.; Authors - Parris Toshima Z TZ
mouse	WB,IF	Cell Rep; Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella; Authors - Xin Zhang
mouse	WB	Cell Mol Life Sci; CCDC146 is required for sperm flagellum biogenesis and male fertility in mice; Authors - Yanjie Ma
mouse	WB,IF	Sci China Life Sci; Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes; Authors - Huan Wu