NME3 Recombinant antibody, PBS Only (Capture)

NME3 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241585H7

Cat no : 84278-5-PBS

Synonyms

NDK 3, KIAA0516, EC:2.7.4.6, DR-nm23, DR nm23

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Validation Data Gallery

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  WB analysis using 84278-5-RR (same clone as 84278-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84278-5-RR (NME3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 84278-5-RR (same clone as 84278-5-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 84278-5-RR (NME3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 84278-5-RR (same clone as 84278-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 84278-5-RR (NME3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 84278-5-RR (same clone as 84278-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 84278-5-RR (NME3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84278-5-RR (same clone as 84278-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NME3 antibody (84278-5-RR, Clone: 241585H7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01200-2

  Cytometric bead array standard curve of MP01200-2, NME3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84278-5-PBS. Detection antibody: 84278-1-PBS. Standard: Ag7284. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84278-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84278-5-RR against Human NME3 were performed. The affinity constant is below 1 pM.

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Product Information

84278-5-PBS targets NME3 as part of a matched antibody pair:

MP01200-2: 84278-5-PBS capture and 84278-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NME3 fusion protein Ag7284
• Full Name: non-metastatic cells 3, protein expressed in
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC000250
• Gene Symbol: NME3
• Gene ID (NCBI): 4832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3), also named as NM23-H3; DR-NM23, is a member of the nucleoside diphosphate kinase (NDPK) family that binds to the mitochondrial outer membrane to stimulate mitochondrial fusion. The depletion of NME3 will cause dysfunction in mitochondrial dynamics. NME3 has been found to facilitate DNA-repair mechanisms binds to several NPHPs, including NEK8, CEP164, and ankyrin repeat and sterile α motif domain-containing 6 (ANKS6)