NME3 Polyclonal antibody

• Featured Product
• KD/KO Validated

NME3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15136-1-AP

Synonyms

NDP kinase 3, NDK 3, KIAA0516, EC:2.7.4.6, DR-nm23

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Validation Data Gallery

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  WB analysis using 15136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15136-1-AP (NME3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15136-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15136-1-AP (NME3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15136-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15136-1-AP (NME3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15136-1-AP

  IP result of anti-NME3 (IP:15136-1-AP, 4ug; Detection:15136-1-AP 1:300) with HEK-293 cells lysate 2400 ug.

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  IHC staining of human breast cancer using 15136-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15136-1-AP (NME3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15136-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15136-1-AP (NME3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15136-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NME3 antibody (15136-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of MCF-7 using 15136-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 15136-1-AP (NME3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, mouse kidney tissue, K-562 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

15136-1-AP targets NME3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NME3 fusion protein Ag7284
• Full Name: non-metastatic cells 3, protein expressed in
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC000250
• Gene Symbol: NME3
• Gene ID (NCBI): 4832
• RRID: AB_10858322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3), also named as NM23-H3; DR-NM23, is a member of the nucleoside diphosphate kinase (NDPK) family that binds to the mitochondrial outer membrane to stimulate mitochondrial fusion. The depletion of NME3 will cause dysfunction in mitochondrial dynamics. NME3 has been found to facilitate DNA-repair mechanisms binds to several NPHPs, including NEK8, CEP164, and ankyrin repeat and sterile α motif domain-containing 6 (ANKS6).

Publications

Species	Application	Title
human,zebrafish	IF,WB	J Biol Chem; The nucleoside diphosphate kinase NME3 associates with nephronophthisis proteins, and is required for ciliary function during renal development.; Authors - Sylvia Hoff ; KD Validated