NME2 Polyclonal antibody, PBS Only

NME2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20493-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse kidney using 20493-1-AP (same clone as 20493-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 20493-1-AP (same clone as 20493-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 20493-1-AP (same clone as 20493-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20493-1-AP (NME2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 20493-1-AP (same clone as 20493-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20493-1-AP (NME2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 20493-1-AP (same clone as 20493-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 20493-1-AP (NME2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20493-1-PBS in a different storage buffer formulation.

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Product Information

20493-1-PBS targets NME2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NME2 fusion protein Ag13913
• Full Name: non-metastatic cells 2, protein (NM23B) expressed in
• Calculated Molecular Weight: 152 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC002476
• Gene Symbol: NME2
• Gene ID (NCBI): 4831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B), NM23B and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates and interacts with membrane receptor and constituting a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing.