NMDAR2A/GRIN2A Recombinant antibody

NMDAR2A/GRIN2A Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240445D4

Cat no : 83465-2-RR

Synonyms

GRIN2A, NMDAR2A, hNR2A, Glutamate receptor ionotropic, NMDA 2A, Glutamate [NMDA] receptor subunit epsilon-1

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Validation Data Gallery

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  WB analysis using 83465-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83465-2-RR (GRIN2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of U-251 using 83465-2-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug Grin2a Recombinant Antibody (83465-2-RR, Clone:240445D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83465-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83465-2-RR against Human NMDAR2A/GRIN2A were performed. The affinity constant is 11.0 nM.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive FC (Intra) detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83465-2-RR is NMDAR2A/GRIN2A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NMDAR2A/GRIN2A fusion protein Ag29101
• Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• Calculated Molecular Weight: 165 kDa
• Observed Molecular Weight: 160-180 kDa
• GenBank Accession Number: NM_000833
• Gene Symbol: GRIN2A
• Gene ID (NCBI): 2903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRIN2A (glutamate ionotropic receptor NMDA type subunit 2A), also known as NMDAR2A. And its molecular weight is 165 kDa. GRIN2A is located in cell projection, dendritic spine, cell membrane, synapse, postsynaptic cell membrae, cytolamic vesicle membrane, which is expressed in many tissues, highest expression in brain and heart. This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without cognitive disability. Alternative splicing results in multiple transcript variants.