neuromedin B Polyclonal antibody

neuromedin B Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10888-1-AP

Synonyms

neuromedin B, NMB

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Validation Data Gallery

• 

  WB analysis of Transfected HEK-293 using 10888-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10888-1-AP (neuromedin B antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10888-1-AP (neuromedin B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10888-1-AP (neuromedin B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10888-1-AP (neuromedin B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10888-1-AP (neuromedin B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10888-1-AP (neuromedin B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human skin using 10888-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10888-1-AP (neuromedin B antibody) at dilution of 1:25 (under 40x lens).

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Tested Applications

• Positive IHC detected in: mouse brain tissue, human brain tissue, human breast cancer tissue, human skin tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

10888-1-AP targets neuromedin B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, monkey, chimpanzee
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: neuromedin B fusion protein Ag0842
• Full Name: neuromedin B
• Calculated Molecular Weight: 16 kDa
• GenBank Accession Number: BC007407
• Gene Symbol: NMB
• Gene ID (NCBI): 4828
• RRID: AB_10895967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuromedin B (NMB) is a bombesin-like peptide, which inhibits food intake and modulates stress-related behaviour. It exerts its function via NMB receptor (NMB-R). NMB is encoded in a 76-amino acid precursor. The mature NMB was reported to be a 10-amino-acid peptide, giving two bands of 1.2 kDa and 1.5 kDa in western blotting. NMB is expressed in brain, gastrointestinal tract, pancreas, adrenals, and adipose tissue. It regulates exocrine and endocrine secretions, cell growth, body temperature and blood pressure and glucose level in vivo.

Publications

Species	Application	Title
chimpanzee	IHC	Brain Struct Funct; The von Economo neurons in frontoinsular and anterior cingulate cortex in great apes and humans.; Authors - Allman John M JM
human	IHC	Cancer Lett; Neuromedin B receptor antagonist suppresses tumor angiogenesis and tumor growth in vitro and in vivo.; Authors - Park Hyun-Joo HJ
human,monkey	WB	Am J Hum Biol; Biochemical specificity of von Economo neurons in hominoids.; Authors - Stimpson Cheryl D CD