NLRP7 Polyclonal antibody
NLRP7 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 27897-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, THP-1 cells |
Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
27897-1-AP targets NLRP7 in WB, IHC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NLRP7 fusion protein Ag27465 |
Full Name | NLR family, pyrin domain containing 7 |
Calculated Molecular Weight | 112 kDa, 115 kDa, 118 kDa |
Observed Molecular Weight | 112 kDa |
GenBank Accession Number | BC109125 |
Gene Symbol | NLRP7 |
Gene ID (NCBI) | 199713 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NLRP7, also called NALP7 or PYPAF3, is a member of the NOD-like receptors (NLR), a family of proteins that plays a crucial role in the innate immune response. NLRP7 has been reported to emerge from NLRP2 gene. Similar to all inflammasomes, NLRP7 contributes to both pro- and an anti-inflammatory processes, depending on whether it functions in an inflammasome-dependent or independent pathway. The function of NLRP7 inflammasome has also been reported to depend on the master regulatory transcription factor protein that controls cellular inflammation, the NF-kB. In addition to its pro- and anti-inflammatory actions, NLRP7 plays a role in restricting intracellular bacterial replication and can also induce inflammasome assembly upon specific stimulation by FSL-1 (diacylated lipoprotein).
Protocols
Product Specific Protocols | |
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WB protocol for NLRP7 antibody 27897-1-AP | Download protocol |
IHC protocol for NLRP7 antibody 27897-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |