NLRP3 Monoclonal antibody

• Featured Product
• KD/KO Validated

NLRP3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

68

CloneNo.

3H1A7

Reviews (1)

Cat no : 68102-1-Ig

Synonyms

EC:3.6.4.-, Cold-induced autoinflammatory syndrome 1 protein, CIAS1, C1orf7, Angiotensin/vasopressin receptor AII/AVP-like

---

Validation Data Gallery

• 

  WB analysis using 68102-1-Ig

  Non-treated THP-1 cells and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 68102-1-Ig (NLRP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

• 

  WB analysis using 68102-1-Ig

  Non-treated RAW 264.7 cells and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68102-1-Ig (NLRP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

• 

  WB analysis using 68102-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68102-1-Ig (NLRP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 68102-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68102-1-Ig (NLRP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 68102-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68102-1-Ig (NLRP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: THP-1 cells, RAW 264.7 cells, LPS and nigericin treated RAW 264.7 cells, LPS treated RAW 264.7 cells, LPS treated THP-1 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

NLRP3 expression is relatively low in normal tissues(PMID: 33538177, 35676979, 25524927, 24166187)

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 53 publications below
• IHC: See 11 publications below
• IF: See 25 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

68102-1-Ig targets NLRP3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, chicken, bovine
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NLRP3 fusion protein Ag26289
• Full Name: NLR family, pyrin domain containing 3
• Calculated Molecular Weight: 118 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_001079821
• Gene Symbol: NLRP3
• Gene ID (NCBI): 114548
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NALP3, also named as C1orf7, CIAS1 and PYPAF1, belongs to the NLRP family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which also known as neonatal onset multisystem inflammatory disease (NOMID).

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Aristolochic acid I exposure triggers ovarian dysfunction by activating NLRP3 inflammasome and affecting mitochondrial homeostasis; Authors - Ming-Xin Sun
mouse	WB	Oxid Med Cell Longev; Hydrogen Sulfide Ameliorated High Choline-Induced Cardiac Dysfunction by Inhibiting cGAS-STING-NLRP3 Inflammasome Pathway; Authors - Lu Bai
human	IF	Front Immunol; USP50 regulates NLRP3 inflammasome activation in duodenogastric reflux-induced gastric tumorigenesis; Authors - Chenye Zhao
human	WB,IF	Cells; Inhibition of Immunoproteasome Attenuates NLRP3 Inflammasome Response by Regulating E3 Ubiquitin Ligase TRIM31; Authors - Yubin Lee
mouse	WB,IF	Int Immunopharmacol; Andrographolide ameliorates sepsis-induced acute lung injury by promoting autophagy in alveolar macrophages via the RAGE/PI3K/AKT/mTOR pathway; Authors - Yuping Qin
human	WB	Front Pharmacol; Bacteroides dorei BDX-01 alleviates DSS-induced experimental colitis in mice by regulating intestinal bile salt hydrolase activity and the FXR-NLRP3 signaling pathway; Authors - Xiaowei Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Marco (Verified Customer) (07-19-2024)

Worked fine.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NRVCM