NLGN1 Monoclonal antibody, PBS Only

NLGN1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D1A12

Cat no : 66964-1-PBS

Synonyms

KIAA1070, neuroligin 1, NLGN1

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Validation Data Gallery

• 

  WB analysis of mouse brain using 66964-1-Ig (same clone as 66964-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66964-1-Ig (same clone as 66964-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit brain using 66964-1-Ig (same clone as 66964-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66964-1-Ig (same clone as 66964-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66964-1-Ig (same clone as 66964-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66964-1-Ig (same clone as 66964-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66964-1-Ig (NLGN1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66964-1-Ig (same clone as 66964-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66964-1-Ig (NLGN1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66964-1-PBS targets NLGN1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NLGN1 fusion protein Ag6356
• Full Name: neuroligin 1
• Calculated Molecular Weight: 840 aa, 94 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC032555
• Gene Symbol: NLGN1
• Gene ID (NCBI): 22871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NLGN1, also named KIAA1070, belongs to the type-B carboxylesterase/lipase family. Neuroligin1 (NLGN1) is a main component of the excitatory glutamatergic synapses complex and plays a role in synapse assembly and function (PMID: 34340665). NLGN1 has 2 isoforms with the MV of 92 and 96 kDa. Sometimes higher molecular weight of around 116 kDa can also be observed, which is a glycosylated mature form of NLGN1 (PMID: 28841651).