SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55484-1-AP (NKX1-2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55484-1-AP (NKX1-2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 55484-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55484-1-AP (NKX1-2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55484-1-AP (NKX1-2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 55484-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55484-1-AP (NKX1-2 Antibody) at dilution of 1:50 (under 40x lens).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:200-1:1000
Immunohistochemistry (IHC)
IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
55484-1-AP targets NKX1-2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55484-1-AP (NKX1-2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 55484-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55484-1-AP (NKX1-2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human brain using 55484-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55484-1-AP (NKX1-2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 55484-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55484-1-AP (NKX1-2 Antibody) at dilution of 1:50 (under 10x lens).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
NKX1-2 Polyclonal antibody
Catalog Number
55484-1-AP
Citations
-
Dilutions
WB : 1:200-1:1000 IHC : 1:20-1:200
Applications
WB, IHC, ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
