NKIRAS2 Polyclonal antibody

NKIRAS2 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 10174-1-AP

Synonyms

Kappa B Ras protein 2, kappaB Ras2, KBRAS2, NKIRAS2

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Validation Data Gallery

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  WB analysis of HEK-293 using 10174-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10174-1-AP (NKIRAS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10174-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10174-1-AP (NKIRAS2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IP experiment of HEK-293 using 10174-1-AP

  IP result of anti-NKIRAS2 (IP:10174-1-AP, 4ug; Detection:10174-1-AP 1:300) with HEK-293 cells lysate 2400 ug.

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  IF Staining of A431 using 10174-1-AP

  Immunofluorescent analysis of A431 cells using 10174-1-AP (NKIRAS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells
• Positive IP detected in: HEK-293 cells
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10174-1-AP targets NKIRAS2 in WB, IP, IF,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NKIRAS2 fusion protein Ag0221
• Full Name: NFKB inhibitor interacting Ras-like 2
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC007450
• Gene Symbol: NKIRAS2
• Gene ID (NCBI): 28511
• RRID: AB_2298444
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

I-Kappa-B-interacting Ras-like protein 2 (KBRAS2, MGC:4553), interact with the PEST domains of IkappaBalpha and IkappaBbeta [inhibitors of the transcription factor nuclear factor kappa B (NF-kappaB)] and decrease their rate of degradation. KBRAS2 differs from other Ras proteins in containing Ala and Leu residues at positions 12 and 61 respectively, which are similar to those present in the oncogenic forms of Ras.

Publications

Species	Application	Title
human	WB	Nat Commun; κB-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis.; Authors - Stephanie Beel

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andrea (Verified Customer) (09-18-2020)

The antibody works great in Western Blot. Good signal and specificity, which was tested on knockout cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: pancreas, different human and mouse cell lines