Anti-Mouse CD314/NKG2D (CX5)

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  FC experiment of C57 mouse splenic NK cells using 65100-1-Ig

  1X10^6 C57 mouse splenic NK cells were surface co-stained with FITC Anti-Mouse CD49b and 0.5 ug Anti-Mouse CD314/NKG2D (65100-1-Ig, Clone: CX5) or unstained (blank control). Cells were not fixed.

CD314/NKG2D Monoclonal Antibody for FC

Cat No. 65100-1-Ig

Clone No.CX5

Host / Isotype

Rat / IgG1, kappa

Reactivity

Mouse

Applications

FC

Synonyms

D6H12S2489E, Klrk1, NKG2 D, Nkg2d, NKG2D (CD314)

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Tested Applications

• Positive FC detected in: C57 mouse splenic NK cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65100-1-Ig targets CD314/NKG2D in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Purified Mouse NKG2D protein
• Full Name: killer cell lectin-like receptor subfamily K, member 1
• GenBank Accession Number: BC057147
• Gene Symbol: CD314
• Gene ID (NCBI): 27007
• RRID: AB_3084899
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: O54709
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In mice, CD314 is expressed on NK cells, activated CD8(+) T cells and macrophages, and subsets of TCR gamma delta (+) and NK1.1 (+) T cells (PMID: 12150888). Various subfamilies of MHC class I-related glycoproteins have been identified as the ligands for mouse CD314, including RAET1A, RAET1B, RAET1C, RAET1D, RAET1E, H60 and MULT1 (PMID: 31720075).