NIT2 Monoclonal antibody, PBS Only

NIT2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E5B1

Cat no : 68169-1-PBS

Synonyms

NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2

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Validation Data Gallery

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  WB analysis of HeLa using 68169-1-Ig (same clone as 68169-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68169-1-Ig (same clone as 68169-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 68169-1-Ig (same clone as 68169-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 68169-1-Ig (same clone as 68169-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 68169-1-Ig (same clone as 68169-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68169-1-PBS targets NIT2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NIT2 fusion protein Ag9889
• Full Name: nitrilase family, member 2
• Calculated Molecular Weight: 276 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC020620
• Gene Symbol: NIT2
• Gene ID (NCBI): 56954
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.