NIT2 Polyclonal antibody

NIT2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16438-1-AP

Synonyms

NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2

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Validation Data Gallery

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  WB analysis using 16438-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16438-1-AP (NIT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 16438-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16438-1-AP (NIT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16438-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16438-1-AP (NIT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16438-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16438-1-AP (NIT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue, human kidney tissue, human brain tissue, human heart tissue, mouse kidney tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

16438-1-AP targets NIT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NIT2 fusion protein Ag9756
• Full Name: nitrilase family, member 2
• Calculated Molecular Weight: 276 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC020620
• Gene Symbol: NIT2
• Gene ID (NCBI): 56954
• RRID: AB_2151317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Mol Biol Rep; NIT2 overexpression predicts poor prognosis in tongue squamous cell carcinoma patients.; Authors - Shan Chen
mouse	WB	Physiol Rep; Effect of NBCe1 deletion on renal citrate and 2-oxoglutarate handling.; Authors - Gunars Osis