Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
11789-1-AP targets NIPSNAP3B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NIPSNAP3B fusion protein Ag2377 Predict reactive species |
| Full Name | nipsnap homolog 3B (C. elegans) |
| Calculated Molecular Weight | 247 aa, 28 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC017914 |
| Gene Symbol | NIPSNAP3B |
| Gene ID (NCBI) | 55335 |
| RRID | AB_2151306 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BS92 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NIPSNAP3B is a member of the NIPSNAP family with putative roles in vesicular trafficking (PMID: 15177564). The gene of NIPSNAP3B maps to chromosome 9q31.3, and encodes a 247-amino-acid protein with a calculated molecular mass of 28 kDa. NIPSNAP3B gene has been reported to be highly associated with low bone mineral density (BMD) variation in postmenopausal Caucasian women (PMID: 19922823).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NIPSNAP3B antibody 11789-1-AP | Download protocol |
| IHC protocol for NIPSNAP3B antibody 11789-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep The clarithromycin-binding proteins NIPSNAP1 and 2 regulate cytokine production through mitochondrial quality control |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kristina (Verified Customer) (01-21-2022) | Beside NIPSNAP3A, I also tested an antibody for NIPSNAP3B as I study these proteins and their relevance in pathophysiology of Parkinson's disease for my PhD thesis. Also the NIPSNAP3B antibody showed a very precise result with one clear band.
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