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NIPA1 Polyclonal antibody

NIPA1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27077-1-AP

Synonyms

FSP3, Magnesium transporter NIPA1, NIPA1, Spastic paraplegia 6 protein, SPG6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NIPA1 Polyclonal antibody (27077-1-AP)

    WB analysis using 27077-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27077-1-AP (NIPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27077-1-AP targets NIPA1 in WB, ELISA applications and shows reactivity with human, Mouse, Rat samples.

Tested Reactivity human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NIPA1 fusion protein Ag23847
Full Name non imprinted in Prader-Willi/Angelman syndrome 1
Calculated Molecular Weight 35 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC025678
Gene Symbol NIPA1
Gene ID (NCBI) 123606
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nonimprinted in Prader-Willi/Angelman loci 1 (NIPA1, also known as SPG6) gene is located in the pericentromeric region of chromosome 15q and encodes a magnesium transporter located in the plasma membrane and early endosomes, implicated in neuronal development and maintenance (PMID: 17166836). NIPA1 is highly conserved along phylogeny and highly expressed in neuronal tissues.

Protocols

Product Specific Protocols
WB protocol for NIPA1 antibody 27077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols