NIPA1 Polyclonal antibody

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  WB analysis using 27077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27077-1-AP (NIPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

NIPA1 Polyclonal Antibody for WB, ELISA

Cat No. 27077-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse, Rat

Applications

WB, ELISA

Synonyms

FSP3, Magnesium transporter NIPA1, NIPA1, Spastic paraplegia 6 protein, SPG6

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27077-1-AP targets NIPA1 in WB, ELISA applications and shows reactivity with human, Mouse, Rat samples.

• Tested Reactivity: human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NIPA1 fusion protein Ag23847
• Full Name: non imprinted in Prader-Willi/Angelman syndrome 1
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC025678
• Gene Symbol: NIPA1
• Gene ID (NCBI): 123606
• RRID: AB_3085923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7RTP0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nonimprinted in Prader-Willi/Angelman loci 1 (NIPA1, also known as SPG6) gene is located in the pericentromeric region of chromosome 15q and encodes a magnesium transporter located in the plasma membrane and early endosomes, implicated in neuronal development and maintenance (PMID: 17166836). NIPA1 is highly conserved along phylogeny and highly expressed in neuronal tissues.