NIP30 Polyclonal antibody

NIP30 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16830-1-AP

Synonyms

PA28G-interacting protein, NEFA-interacting nuclear protein NIP30, FAM192A, CDA10, CDA018

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Validation Data Gallery

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  WB analysis of HeLa using 16830-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16830-1-AP (NIP30 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16830-1-AP

  IP result of anti-NIP30 (IP:16830-1-AP, 4ug; Detection:16830-1-AP 1:500) with HeLa cells lysate 1080ug.

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  IF Staining of HeLa using 16830-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16830-1-AP (NIP30 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16830-1-AP targets NIP30 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NIP30 fusion protein Ag10439
• Full Name: NEFA-interacting nuclear protein NIP30
• Calculated Molecular Weight: 254 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC071952
• Gene Symbol: NIP30
• Gene ID (NCBI): 80011
• RRID: AB_2109703
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NIP30, also known as NEFA-interacting nuclear protein 30, FAM192A (family with sequence similarity 192, member A), is a 254 amino acid nuclear protein encoded by a gene that maps to human chromosome 16q13.

Publications

Species	Application	Title
human	WB	Nat Commun; The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells.; Authors - Xiao Gao
human	WB	Retrovirology; Comparative host protein interactions with HTLV-1 p30 and HTLV-2 p28: insights into difference in pathobiology of human retroviruses.; Authors - Doueiri Rami R