Nidogen 2 Polyclonal antibody

Nidogen 2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13530-1-AP

Synonyms

NID 2, NID2, Nidogen 2, nidogen 2 (osteonidogen), Osteonidogen

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Validation Data Gallery

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  WB analysis of human placenta using 13530-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13530-1-AP (Nidogen 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13530-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13530-1-AP (Nidogen 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13530-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13530-1-AP (Nidogen 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, human placenta tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human kidney tissue, mouse skeletal muscle tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13530-1-AP targets Nidogen 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nidogen 2 fusion protein Ag4424
• Full Name: nidogen 2 (osteonidogen)
• Calculated Molecular Weight: 1375 aa, 151 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC035608
• Gene Symbol: NID2
• Gene ID (NCBI): 22795
• RRID: AB_10598468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nidogen, also referred to as entactin, represents a highly conserved protein in vertebrates and invertebrates and is found in almost all basement membranes (BM). Nidogen-1 (150 kDa) and nidogen-2 (200 kDa) are elongated molecules composed of three globular domains (G1, G2, and G3) connected by a flexible, protease-sensitive link and a rigid rod-like domain. Western blot analysis of protein extracts of heart, lung, and skeletal muscle of mice, major bands of about 200 kDa were detected, accompanied by smaller and variable bands of 160-170 kDa. (PMID: 12215539)

Publications

Species	Application	Title
human	IHC	Front Cell Dev Biol; Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis.; Authors - Mansheng Li
human	WB,IF	Int Immunopharmacol; Identification of TAP1 as a T-cell related therapeutic target in gastric cancer by mediating oxalipliatin-related synergistic enhancement of immunotherapy; Authors - Yupeng Zhao
mouse	WB	bioRxiv; Insulin-Independent Regulation of Type 1 Diabetes via Brown Adipocyte-Secreted Proteins and the Novel Glucagon Regulator Nidogen-2; Authors - Jeongmin Lee