NHP2 Polyclonal antibody

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  WB analysis of SH-SY5Y using 15128-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 15128-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15128-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15128-1-AP

  IP result of anti-NHP2 (IP:15128-1-AP, 4ug; Detection:15128-1-AP 1:500) with HepG2 cells lysate 3600ug.

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  IHC staining of human prostate cancer using 15128-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15128-1-AP (NHP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human prostate cancer using 15128-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15128-1-AP (NHP2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 15128-1-AP

  Immunofluorescent analysis of HepG2 cells, using NHP2 antibody 15128-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

NHP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15128-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

NOLA2, NHP2P, NHP 2, HSPC286

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, Caco-2 cells, HeLa cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

15128-1-AP targets NHP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NHP2 fusion protein Ag7251
• Full Name: NHP2 ribonucleoprotein homolog (yeast)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17-20 kDa
• GenBank Accession Number: BC000009
• Gene Symbol: NHP2
• Gene ID (NCBI): 55651
• RRID: AB_2267290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NX24
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NHP2 belongs to the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. It is required for ribosome biogenesis and telomere maintenance, and for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.

Publications

Species	Application	Title
human	WB	Nature; Structure of human telomerase holoenzyme with bound telomeric DNA.; Authors - George E Ghanim
human	WB	Nature; Cryo-EM structure of substrate-bound human telomerase holoenzyme.; Authors - Thi Hoang Duong Nguyen
human	WB,IF	EMBO J; NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.; Authors - Maya Raghunandan ; KD Validated
human,yeast	WB	EMBO Rep; Nhp2 is a reader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis.; Authors - Julia S P Mawer
mouse	IHC	Biomedicines; Comparative Proteomic Analysis of tPVAT during Ang II Infusion.; Authors - Xiuying Liang
human	WB	Mol Cell Biol; SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization.; Authors - D E MacNeil