SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Caco-2 using 15128-1-AP
Caco-2 cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Caco-2 cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15128-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 15128-1-AP
IP result of anti-NHP2 (IP:15128-1-AP, 4ug; Detection:15128-1-AP 1:500) with HepG2 cells lysate 3600ug.
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human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
NHP2 belongs to the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. It is required for ribosome biogenesis and telomere maintenance, and for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Caco-2 using 15128-1-AP
Caco-2 cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15128-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human prostate cancer using 15128-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15128-1-AP (NHP2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human prostate cancer using 15128-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15128-1-AP (NHP2 antibody) at dilution of 1:100 (under 10x lens).
IP Figures
IP experiment of HepG2 using 15128-1-AP
IP result of anti-NHP2 (IP:15128-1-AP, 4ug; Detection:15128-1-AP 1:500) with HepG2 cells lysate 3600ug.
IF/ICC Figures
IF Staining of HepG2 using 15128-1-AP
Immunofluorescent analysis of HepG2 cells, using NHP2 antibody 15128-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
NHP2 Polyclonal antibody
Catalog Number
15128-1-AP
Citations
16
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, yeast
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet