NHE1 Polyclonal antibody

NHE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 29761-1-AP

Synonyms

SLC9A1, NHE 1, Na(+)/H(+) exchanger 1, Na(+)/H(+) antiporter, amiloride-sensitive, FLJ42224

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Validation Data Gallery

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  WB analysis using 29761-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29761-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 29761-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 29761-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 29761-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NHE1 antibody (29761-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29761-1-AP targets NHE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NHE1 fusion protein Ag31192
• Full Name: solute carrier family 9 (sodium/hydrogen exchanger), member 1
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: NM_003047
• Gene Symbol: NHE1
• Gene ID (NCBI): 6548
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NHE1, also named as SLC9A1, encodes an Na+/H+ antiporter that is a membrane-bound enzyme involved in regulating pH of vertebrate cells. NHE1 regulates a number of cell behaviors, including adhesion, shape determination, migration, and proliferation (PMID:11807182). It is inhibited by the non-specific diuretic drug amiloride and activated by growth factors, mitogens, neurotransmitters, tumor promoters, and others (PMID: 2846238). NHE1 is expressed in heart where it contributes to cardiomyocyte pH homeostasis (PMID: 12832126, PMID: 23709596).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katja (Verified Customer) (06-05-2024)

test in CHO cells parental (lane 1) and NHE1 knockout CHO cells (lane 2)

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: CHO

NHE1 Antibody Western Blot validation (1:2000 dilution) in CHO (Cat no:29761-1-AP)