Various lysates were subjected to SDS PAGE followed by western blot with 30229-1-AP (NGEF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 30229-1-AP (NGEF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 30229-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 30229-1-AP (NGEF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 30229-1-AP (NGEF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
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PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
NGEF enables guanyl-nucleotide exchange factor activity. Predicted to be involved in several processes, including activation of GTPase activity; ephrin receptor signaling pathway; and negative regulation of dendritic spine morphogenesis.
Various lysates were subjected to SDS PAGE followed by western blot with 30229-1-AP (NGEF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 30229-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 30229-1-AP (NGEF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
