NGDN Polyclonal antibody

NGDN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16524-1-AP

Synonyms

LCP5, EIF4E-binding protein, EIF4E binding protein, Centromere accumulated nuclear protein 1, CANu1

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Validation Data Gallery

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  WB analysis of human cerebellum using 16524-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16524-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human epididymis using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human epididymis using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16524-1-AP

  Immunofluorescent analysis of Hela cells, using NGDN antibody 16524-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: human cerebellum tissue, mouse brain tissue, mouse testis tissue
• Positive IHC detected in: human epididymis tissue, human heart tissue, human kidney tissue, human liver tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16524-1-AP targets NGDN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NGDN fusion protein Ag9763
• Full Name: neuroguidin, EIF4E binding protein
• Calculated Molecular Weight: 315 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC030817
• Gene Symbol: NGDN
• Gene ID (NCBI): 25983
• RRID: AB_2152270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	Nature; A viral Sm-class RNA base-pairs with mRNAs and recruits microRNAs to inhibit apoptosis.; Authors - Carlos Gorbea