NGAL Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NGAL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 26991-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human saliva using 26991-1-AP (same clone as 26991-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 26991-1-AP (same clone as 26991-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 26991-1-AP (same clone as 26991-1-PBS)

  WB result of NGAL antibody (26991-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells. This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 26991-1-AP (same clone as 26991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 26991-1-AP (same clone as 26991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26991-1-AP (LCN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 26991-1-AP (same clone as 26991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 26991-1-AP (same clone as 26991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26991-1-AP (LCN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 26991-1-AP (same clone as 26991-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Lipocalin-2/NGAL antibody (26991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 26991-1-AP (same clone as 26991-1-PBS)

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Lipocalin-2/NGAL (26991-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

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Product Information

26991-1-PBS targets NGAL in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NGAL fusion protein Ag25715
• Full Name: lipocalin 2
• Observed Molecular Weight: 25 kDa
• Gene Symbol: NGAL/LCN2
• Gene ID (NCBI): 3934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).