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NFkB p65 antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Human

Applications

ChIP, ChIP-Seq, EMSA, WB

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Cat No : 39369,39370 39369

Synonyms

NFkB, p65, NFkB p65, NF KB, NF-kB, NF-kB p65, NF-kappaB, NF kappa B, pAb, polyclonal, NF-kappa-B, RelA, antibody, antibodies, sample, dbdabs

Validation Data Gallery

  • NFkB p65 antibody tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 25 ug of chromatin from a human brain tumor and 10 ul of NFkB p65 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 9.5 million sequence tags were mapped to identify NFkB p65 binding sites on chromosome 19.

    NFkB p65 antibody tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 25 ug of chromatin from a human brain tumor and 10 ul of NFkB p65 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 9.5 million sequence tags were mapped to identify NFkB p65 binding sites on chromosome 19.

  • NFkB p65 (pAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 2 million HT29 cells treated with TNF and 10 ul of p65 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

    NFkB p65 (pAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 2 million HT29 cells treated with TNF and 10 ul of p65 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

  • NFkB p65 (pAb) tested by Western blot. NFkB p65 detection by Western blot. The analysis was performed using 20 ug HeLa cytoplasmic extract and NFkB p65 pAb at a 1:2,500 dilution.

    NFkB p65 (pAb) tested by Western blot. NFkB p65 detection by Western blot. The analysis was performed using 20 ug HeLa cytoplasmic extract and NFkB p65 pAb at a 1:2,500 dilution.

Product Information

Tested Applications ChIP, ChIP-Seq, EMSA, WB

Applications Validated by Active Motif: ChIP-Seq: 10 ul per ChIP ChIP: 4 ul per ChIP WB: 1:2,500 - 1:5,000 dilution
Tested Reactivity Human
Host / Isotype Rabbit / Serum
Class Polyclonal
Type Antibody
Immunogen This NFkB p65 antibody was raised against a peptide immunogen corresponding to the C-terminal of human p65.
Full Name NFkB p65 antibody (pAb)
Synonyms NFkB, p65, NFkB p65, NF KB, NF-kB, NF-kB p65, NF-kappaB, NF kappa B, pAb, polyclonal, NF-kappa-B, RelA, antibody, antibodies, sample, dbdabs
Molecular weight 65 kDa
GenBank accession numberNP_068810
RRIDAB_2793231
Purification Method None
Buffer Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

NFkB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFkB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFkB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFkB p50 (proteolytically processed from p105/NFkB1) and p52 (proteolytically processed from p100/NFkB2) members of the NFkB family that bind DNA and activate transcription. The NFkB p65/p50 heterodimer is the most abundant form of NFkB. NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB. The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). Upon its activation, the IKK complex phosphorylates IkB proteins (IkBa, IkBa phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFkB complex to translocate to the nucleus where it regulates transcription by binding to DNA.