IkB Alpha Polyclonal antibody

IkB Alpha Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 18220-1-AP

Synonyms

IKBA, IκB α, NFKBIA, Ikβ-α, IkB-alpha

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Validation Data Gallery

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  WB analysis of HL-60 using 18220-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 18220-1-AP (IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18220-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18220-1-AP (IkB alpha antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18220-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18220-1-AP (IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18220-1-AP

  IP result of anti-IkB Alpha (IP:18220-1-AP, 5ug; Detection:18220-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human kidney using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18220-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18220-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18220-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18220-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18220-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18220-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18220-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 18220-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18220-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 18220-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18220-1-AP (IkB alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 18220-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IkB Alpha antibody (18220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human liver tissue, human prostate cancer tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below

Product Information

18220-1-AP targets IkB Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IkB Alpha fusion protein Ag12979
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC004983
• Gene Symbol: IkB Alpha
• Gene ID (NCBI): 4792
• RRID: AB_2298441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Publications

Species	Application	Title
human	WB	Mol Cancer; Activation of the NF-¿B pathway as a mechanism of alcohol enhanced progression and metastasis of human hepatocellular carcinoma.; Authors - Fei Wang
rat	WB	Front Mol Neurosci; Electroacupuncture Suppresses the NF-κB Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats.; Authors - Jin Jiang
rat	WB	J Neuroinflammation; Upregulation of neuronal zinc finger protein A20 expression is required for electroacupuncture to attenuate the cerebral inflammatory injury mediated by the nuclear factor-kB signaling pathway in cerebral ischemia/reperfusion rats.; Authors - Jian Zhan
mouse	WB	Biomed Pharmacother; Tubeimoside I improves survival of mice in sepsis by inhibiting inducible nitric oxide synthase expression.; Authors - Minghao Luo
human	WB	Cancer Biol Ther; MiR-376a-3p increases cell apoptosis in acute myeloid leukemia by targeting MT1X.; Authors - Xiangke Xin
mouse,rat	WB	Eur J Pharmacol; Tubeimoside I protects against sepsis-induced cardiac dysfunction via SIRT3.; Authors - Zhe Cheng