NFKB1,p105 Polyclonal antibody

NFKB1,p105 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23576-1-AP

Synonyms

NFKB1, NF kappa B, KBF1, EBP-1, EBP 1

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 23576-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 23576-1-AP (NFKB1,p105 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23576-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23576-1-AP (NFKB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 23576-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23576-1-AP (NFKB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 23576-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23576-1-AP (NFKB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 23576-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 23576-1-AP (NFKB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 23576-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23576-1-AP (NFKB1,p105 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 23576-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23576-1-AP (NFKB1,p105 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 23576-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 23576-1-AP (NFKB1,p105 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells, Jurkat cells, K-562 cells, Raji cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• ChIP: See 1 publications below

Product Information

23576-1-AP targets NFKB1,p105 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFKB1,p105 fusion protein Ag20297
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC051765
• Gene Symbol: NFKB1
• Gene ID (NCBI): 4790
• RRID: AB_2879299
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind p105 isoforms of NFKB1.

Publications

Species	Application	Title
human	WB	Oncotarget; Single cell whole genome sequencing reveals that NFKB1 mutation affects radiotherapy sensitivity in cervical cancer.; Authors - Dong Yang
human	ChIP	In Vitro Cell Dev Biol Anim; NFKB1-miR-612-FAIM2 pathway regulates tumorigenesis in neurofibromatosis type 1.; Authors - Meng Wang