NFIA Monoclonal antibody

• Featured Product
• KD/KO Validated

NFIA Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig, rabbit and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3A6D7

Cat no : 66999-1-Ig

Synonyms

CTF, DKFZp434L0422, DKFZp686J23256, FLJ39164, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein

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Validation Data Gallery

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  WB analysis using 66999-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of MCF-7 using 66999-1-Ig

  WB result of NFIA antibody (66999-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFIA transfected MCF-7 cells.

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  WB analysis of HeLa using 66999-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66999-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 66999-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 66999-1-Ig

  IP result of anti-NFIA (IP:66999-1-Ig, 5ug; Detection:66999-1-Ig 1:500) with HeLa cells lysate 1600 ug.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse cerebellum tissue, fetal human brain tissue, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66999-1-Ig targets NFIA in WB, IP, ELISA applications and shows reactivity with Human, rat, mouse, pig, rabbit samples.

• Tested Reactivity: Human, rat, mouse, pig, rabbit
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NFIA fusion protein Ag17873
• Full Name: nuclear factor I/A
• Calculated Molecular Weight: 498 aa, 55 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC022264
• Gene Symbol: NFIA
• Gene ID (NCBI): 4774
• RRID: AB_2882316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)

Publications

Species	Application	Title
human	WB	Sci Transl Med; Single-cell multiomics guided mechanistic understanding of Fontan-associated liver disease; Authors - Po Hu