NFIA Polyclonal antibody

NFIA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 11750-1-AP

Synonyms

CTF, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein

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Validation Data Gallery

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  WB analysis of A431 using 11750-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 11750-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11750-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11750-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11750-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 11750-1-AP

  IP result of anti-NFIA (IP:11750-1-AP, 4ug; Detection:11750-1-AP 1:500) with A431 cells lysate 2000ug.

• 

  IHC staining of mouse brain using 11750-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11750-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11750-1-AP (NFIA antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human gliomas using 11750-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11750-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11750-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, Jurkat cells, L02 cells, mouse liver tissue
• Positive IP detected in: A431 cells
• Positive IHC detected in: mouse brain tissue, human prostate cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

11750-1-AP targets NFIA in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFIA fusion protein Ag2346
• Full Name: nuclear factor I/A
• Calculated Molecular Weight: 498 aa, 55 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC022264
• Gene Symbol: NFIA
• Gene ID (NCBI): 4774
• RRID: AB_2153214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. Catalog# 11750-1-AP is a rabbit polyclonal antibody raised against N-terminal of human original NFIA. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)

Publications

Species	Application	Title
human	WB	Cell Cycle; TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs.; Authors - Daniel J Foster
human	WB	Cancer Cell Int; Macrophages-derived exosomal lncRNA LIFR-AS1 promotes osteosarcoma cell progression via miR-29a/NFIA axis.; Authors - Hongliang Zhang
mouse	IHC	FEBS Lett; STAT3 transcriptionally regulates the expression of genes related to glycogen metabolism in developing motor neurons; Authors - Hitoshi Gotoh
human	WB,CoIP	Pathol Res Pract; SOX9/NFIA promotes human ovarian cancer metastasis through the Wnt/β-catenin signaling pathway; Authors - Rong Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

KUN (Verified Customer) (01-13-2021)

Works for western blot, recogizning both NFIA and also other NFI family proteins

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T