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NFATC3 Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1

Detection: Mouse / IgG1

Reactivity

Capture: Human

Detection: Human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.625-20 ng/mL

Cat No : MP50022-1

Print datasheet

Synonyms

NF AT4, NF ATc3, NFAT4, NFATC3, NFATX

Validation Data Gallery

  • Sandwich ELISA standard curve ofMP50022-1, NFATC3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66924-2-PBS. Detection antibody: HRP-conjugated 66924-1-PBS. Standard: Ag27306. Range: 0.625-20 ng/mL.

    Sandwich ELISA standard curve of MP50022-1

    Sandwich ELISA standard curve ofMP50022-1, NFATC3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66924-2-PBS. Detection antibody: HRP-conjugated 66924-1-PBS. Standard: Ag27306. Range: 0.625-20 ng/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP50022-1 targets NFATC3 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.625-20 ng/mL (Sandwich ELISA);

Capture antibody: NFATC3 Monoclonal antibody, PBS Only (Capture) 66924-2-PBS (1C3B10). 100 μg. Concentration 1 mgl/ml.

Detection antibody: NFATC3 Monoclonal antibody, PBS Only (Detector) 66924-1-PBS (1C1H8). 100 μg. Concentration 1 mgl/ml.

Matched pair protein standard: Ag27306

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 66924-2-PBS
Tested Reactivity Human
Immunogen NFATC3 fusion protein Ag27306
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=5.73 x 10-11M
KOff=4.88 x 10-6M
KOn=8.53 x 104M
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
Synonyms NF AT4, NF ATc3, NFAT4, NFATC3, NFATX
GenBank accession numberBC001050
Gene symbol NFATC3
UniprotQ12968
Gene ID (NCBI) 4775
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 66924-1-PBS
Tested Reactivity Human
Immunogen NFATC3 fusion protein Ag27306
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.16 x 10-10M
KOff=5.67 x 10-6M
KOn=4.90 x 104M
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
Synonyms NF AT4, NF ATc3, NFAT4, NFATC3, NFATX
GenBank accession numberBC001050
Gene symbol NFATC3
UniprotQ12968
Gene ID (NCBI) 4775
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.