NFATC2IP Polyclonal antibody

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  WB analysis using 11667-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11667-1-AP (NFATC2IP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 11667-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFATC2IP antibody (11667-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

NFATC2IP Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 11667-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Nuclear factor of activated T-cells, cytoplasmic 2-interacting protein, NIP45, NFATC2-interacting protein, NFATC2 interacting protein, 45 kDa NFAT-interacting protein

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, Raji cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11667-1-AP targets NFATC2IP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFATC2IP fusion protein Ag2217
• Full Name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein
• Calculated Molecular Weight: 138 aa, 15 kDa
• Observed Molecular Weight: 50-65 kDa
• GenBank Accession Number: BC021551
• Gene Symbol: NFATC2IP
• Gene ID (NCBI): 84901
• RRID: AB_3669129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NCF5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFATC2IP, also named as NIP45, is a 419 amino acid protein, which contains one ubiquitin-like domain. TRAF1 is associated with a fraction of NFATC2IP in the cytoplasm and prevents its translocation to the nucleus. NFATC2IP In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2.