NF90/ILF3 Polyclonal antibody

• Featured Product
• KD/KO Validated

NF90/ILF3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

29

Reviews (2)

Cat no : 19887-1-AP

Synonyms

ILF3, M phase phosphoprotein 4, IL-F3, DRBP76, DRBF

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Validation Data Gallery

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  WB analysis using 19887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19887-1-AP (ILF3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19887-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19887-1-AP (ILF3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 19887-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19887-1-AP (NF90,ILF3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19887-1-AP

  IP result of anti-NF90/ILF3 (IP:19887-1-AP, 3ug; Detection:19887-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of human cervical cancer using 19887-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19887-1-AP (ILF3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 19887-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19887-1-AP (ILF3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 19887-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19887-1-AP (ILF3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19887-1-AP

  Immunofluorescent analysis of Hela cells, using ILF3 antibody 19887-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, mouse spleen tissue, K-562 cells, Raji cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 25 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below
• RIP: See 7 publications below

Product Information

19887-1-AP targets NF90/ILF3 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NF90/ILF3 fusion protein Ag13707
• Full Name: interleukin enhancer binding factor 3, 90kDa
• Calculated Molecular Weight: 894 aa, 95 kDa
• Observed Molecular Weight: 90 kDa, 110 kDa
• GenBank Accession Number: BC064836
• Gene Symbol: ILF3
• Gene ID (NCBI): 3609
• RRID: AB_10666431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor of activated T cells (NFAT)is a transcription factor required for T-cell expression of interleukin-2 (IL2). NFAT binds to a sequence known as the antigen receptor response element-2 (ARRE2) in the IL2 enhancer. NFAT contains two subunits, one is ILF3. ILF3 can facilitate double-standed RNA-regulated gene expression at the level of post-transcription. It binds to and inhibits coding sequences of acid beta-glucosidase and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 exists main two isoforms NFAR-1 (90 kDa) and -2 (110 kDa). NFAR-1 and -2 each contain two DRBDs in their C-terminus regions and exhibit 98% homology to one another at the amino acid level. NFAR-2 contains an extra 192 amino acids at the C-terminal region that are encoded by an extra three exons present in its corresponding transcript.

Publications

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun ; KD Validated
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu
human	WB	EMBO J; Suppression of miR-199a maturation by HuR is crucial for hypoxia-induced glycolytic switch in hepatocellular carcinoma.; Authors - Ling-Fei Zhang
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
mouse	RIP	Cancer Res; CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942.; Authors - Chi Hin Wong
human	WB	Nucleic Acids Res; Oxygen-dependent expression of cytochrome c oxidase subunit 4-2 gene expression is mediated by transcription factors RBPJ, CXXC5 and CHCHD2.; Authors - Aras Siddhesh S

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Praveen (Verified Customer) (03-13-2019)

Very Good

• Applications: Western Blot,
• Cell Tissue Type: HEK293

NF90/ILF3 Antibody Western Blot, validation ( dilution) in HEK293 (Cat no:19887-1-AP)

FH

SHUBHAM (Verified Customer) (02-25-2019)

eXCELLENT

• Applications: Western Blot,
• Cell Tissue Type: Ac16

NF90/ILF3 Antibody Western Blot, validation ( dilution) in Ac16 (Cat no:19887-1-AP)